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Scherrer, S./ Zippler, U./ Honegger, R. 2005: Characterisation of the mating-type locus in the genus Xanthoria (lichen-forming ascomycetes, Lecanoromycetes). - Fungal Genetics and Biology 42: 976-988. [RLL List # 201 / Rec.# 28124]
Keywords: MATING TYPES/ XANTHORIA/ GENETICS/ MAT LOCUS/ α-BOX FRAGMENT/ α-DOMAIN/ HMG BOX/ HETEROTHALLIC/ HOMOTHALLIC/ IDIOMORPH/ SINGLE SPORE ISOLATES
Abstract: [Authors used Xanthoria parietina, X. polycarpa, X. flammea and X. elegans. "The complete MAT locus, containing one ORF (MAT-2-1) coding for a truncated HMG-box protein, and two partial flanking genes, were cloned by screening a genomic lambda phage library of the homothallic X. parietina. The occurrence of mating-type genes in eight single spore isolates derived from one ascus was studied with a PCR assay. In the homothallic X. parietina a HMG fragment, but no α-box fragment was found in all isolates, whereas in X. elegans, another homothallic species, all tested isolates contained a fragment of both idiomorphs. Conversely, isolates of the heterothallic X. polycarpa contained either a HMG or an α-box fragment, but never both."

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